Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Semaphorin 3F (also known as SEMA3F) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SEMA3F antibody. SEMA3F present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SEMA3F Antibody is added and binds to SEMA3F in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA3F antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SEMA3F. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm